Fig. 2
From: sRNAPipe: a Galaxy-based pipeline for bioinformatic in-depth exploration of small RNAseq data
Examples of results obtained from sRNAPipe. a Length distribution of the reads. b Mapping to categories of genomic sequences. The pie chart shows percentages of reads of the chosen size range (here 18–32 nt) mapping to microRNAs (miRNAs), gene transcripts (“transcripts”), small nuclear RNAs (snRNAs), ribosomal RNAs (rRNAs), transfer RNAs (tRNAs), transposable elements (TEs) and other, non-annotated sequences (“others”). c Mapping of bonafide piRNAs, 23–29 nt long, along genomic coordinates of chromosome 2R. Reads mapped to the Plus strand are in blue, those mapped to the minus strand in red. d Mapping of genome-unique bonafide piRNAs, 23–29 nt long, along genomic coordinates of chromosome 2R. Colors as in C. e Density profile of putative piRNA coverage (23–29 nt) along the FW LINE-like retrotransposon, mapped allowing 0 to 3 mismatches. Colors as in C. f Histogram of the percentage of 5′-overlaps of sense and antisense read of the FW-mapping piRNAs (23–29 nt)