Table 2 Primers for amplifying mtDNA fragments for genotyping in this study
Primer pair name | Primer sequence (5’→3’) | Gene name and location of primer |
|---|---|---|
ND2F | CAAGCTGCACCATTCCATA | ND2 |
ND2R | CCATTAGTGGTGGTACTCC | ND2 |
ND4F | GGGAGAATTTGATTCAAGTGCAAC | ND4 |
ND4R | CATACATGGAAAGGTACTAG | ND4 |
Da20 | GACACTACACAAGATGCCTC | COX1 exon 1 |
Da3 | GCAATAGCATATACCATCCCG | COX1 exon 3 |
Da22 | CTCGAGCTTACTTTACAGCAG | COX1 exon 3 |
Da19 | GTACTACTCCTGTTAGTCCTC | COX1 exon 4 |
Da26 | CAACGGCATACGGTGGATCTATCC | COX1 exon 4 |
Da15 | CTGTTAGATATGATGGTGTGC | COX1 exon 6 |
COB1F | CCACAACCTATTAACATTAGCTACGC | COB1 exon 1 |
COB1R | CGTCTCCATCTACAAAGCCAGCAAAC | COB1 Intron 2 |
ND5F | CTATTGGTGTTACAGGAGCTCAC | ND5 |
ND5R | GAGCCTTCATACCTGCCTTATTTGC | ND5 |
LsrRNAF | CAGCAGAACCCTTCCCAGC | Upstream LsrRNA |
LsrRNAR | CCTCCACTGTCTCATGCGG | LsrRNA exon 3 |
Cox1F | TGTCTGGAGCTGGTAACCAAT | COX1 exon 1 |
Cox1R | AAGAGGTGTTCATATAGAACTGG | COX1 exon 1 |
ATP6F | GACACACTTTATTACATCTCCAC | ATP6 |
ATP6R | GAAGTTCAATGGCATCCTTG | ATP6 |
N2A8F | AACTCCCCACATAGTTATGG | ND2-ATP8ig a |
N2A8R | CATCCCTGTTATTAATTCACT | ND2-ATP8 ig |
ATP8F | TTTCAATGGGTGCTGTGTTC | ATP8-COX1ig b |
ATP8R | CCGAATGTAATTTGGTTTACCC | ATP8 |
ATP9F | CGGACTATCAGGAGCTGGAG | ATP9 |
ATP9R | TTGGTGGTCACCGTTTAGAA | ATP9-COX1ig c |
ND6F | ACTTGATCTTCTTGCATTTGG | ND6 |
ND6R | TTATGTTCGTGGTCGTAGACA | ND6 |