Deletion of the sex-determining gene SXI1α enhances the spread of mitochondrial introns in Cryptococcus neoformans

Table 1 Parental strains used in this study and their genotypes

Strain	Genotype^a	Reference
CHY618	MATα ura5 mtD sxi1α::NAT	Hull et al. 2002
CHY620	MATα ura5 mtD SXI1α NAT	Hull et al. 2002
CHY647	MATα ura5 mtD sxi1α::NAT URA5 ectopic pPM8 vector	Hull et al. 2002
CHY648	MATα ura5 mtD sxi1α::NAT URA5 ectopic pPM8-SXI1α	Hull et al. 2002
YZX2	MATa ade2 mtA NEO	Yan et al. 2004

^aThe above five strains are isogenic except at the indicated loci. MATa: mating type a; MATα: mating type α. Strains with ura5 or ade2 auxotrophic markers require uracil or adenine respectively for growth on the minimum SD medium. mtA and mtD refer to the two parental mitochondrial genotypes distinguished by polymorphisms at loci ND2, ND4, ND5 and COX1. mtD contains one and four extra introns in ND5 and COX1 respectively compared to mtA in strain YZX2. For details of alleles at these three loci as well as at the COX1 locus, please see the main text, Fig. 1, Table 3, and references Xu (2002) and Yan and Xu (2003)

ISSN: 1759-8753