Fig. 3

Expression of P-element and KP-element mRNAs in ovaries and their relationships to GD scores (a) Fractions of silent- and active-expression regions that harbor P elements in the respective genomes. The fractions were calculated by dividing the number of sequencing reads that supported insertion of the respective regions by the total sequencing reads to represent occupancy-adjusted copy numbers. 1% represents the fraction of unknown region that harbors P elements in the respective genomes. b, c The levels of P-element mRNA (b) and KP-element mRNA (c) in young F1 ovaries. The expressions were quantified by qRT-PCR and normalized by those of M′-OM5 hybrids using qRT-PCR. Star (*) indicates statistical significance (p < 0.05). d, e The relationship between the level of KP-element mRNAs in F1 ovaries (x-axis) and GD scores (y-axis) of F1 (d) and F2 (e) progenies