Fig. 2

Genomic composition of P elements (a) Top: Results of PCR analysis amplifying total P elements (left, labeled with total P on the top) and non-KP elements (right, labeled with non-KP) using the respective genomic DNAs indicated on the top of each lane. The positions of DNA bands derived from FP (2526 bp) and KP (789 bp) elements produced in the “total P” PCR are indicated on the left. The positions of bands derived from the FP (2206 bp) and incomplete (internally deleted) elements produced in the “non-KP” PCR are indicated on the right. Bottom: Structure of the FP and KP elements and the primer design used for the PCR. The deletion junctions (at 808 and 2560 bp) of the KP element are indicated. b The copy numbers of P elements in the respective genomes relative to that in the M′-OM5 genome. Star (*) indicates statistical significance (p < 0.05). c The ratio of KP elements to the total P-element copies quantified by qPCR with KP- and total-P-specific primers