Table 1 Plasmids and primers used in this study^a

Genotype/Description

 pET28-ISSau2

pET28b derivate with ISSau2 transposase sequence under control of T7 promoter, Kan^R

 pET32-OrfAB

pET32a derivate with ISSau2 transposase sequence with an insertion of G residue in the A₆G₁ region to generate the A₆G₂ region, Amp^R

 pET32a-OrfA

pET32a derivate with OrfA sequence (the first orf of ISSau2, 753 bp in length), Amp^R

 pET32a-OrfB

pET32a derivate with OrfB sequence (the second orf of ISSau2, 891 bp in length), Amp^R

 pUC19-IRL-gfp-IRR

pUC19 derivate with the gfp sequence from pGLO vector flanked by IRL (42 bp) and IRR (42 bp) of ISSau2, Amp^R

 pET28a-ISgfp

pET28a derivate with the gfp sequence from pGLO vector flanked by IRL (42 bp) and IRR (42 bp) of ISSau2, Kan^R

Primer

Sequence 5′-3’

 ISSau2-F

CGCGGATCCATGAAAACTTTGAAGGGAGC

 ISSau2-R

CCGCTCGAGCATTAAAAATGGCTGATTCTGTA

 OrfAB-overlap-R

ATGCTTCAAACCTTTTTTAAAACGT

 OrfAB-overlap-F

ACGTTTTAAAAAAGGTTTCAAGCAT

 OrfA-R

CCGCTCGAGACGCTTCAATCGTTTTGTAT

 OrfB-F

CGCGGATCCCGTTTTAAAAAAGTTTCAAGCA

 IRL-gfp-F

CGCGGATCCTGAAATGCTCCCTTCAAAGTAGACATTGAAAAAATGAAAACTAT

GGCTAGCAAAGGAGAA

 IRR-gfp-R

CCGGAATTCAATTTTAATTATTTAATTGTCTACTAGACTGGGTGCAGTTCATTTG

TAGAGCTCATCCAT

 D-orfA-F

ATGAAAACTTTGAAGGGAGC

 D-orfA-R

ACGCTTCAATCGTTTTGTAT

 D-orfB-F

CGTTTTAAAAAAGTTTCAAGCA

 D-orfB-R

CATTAAAAATGGCTGATTCTGTA

 D-ISgfp-F

AATGAAAACTATGGCTAGCAA

 D-ISgfp-R

TGCAGTTCATTTGTAGAGCT