Fig. 5
From: Characterization of a functional insertion sequence ISSau2 from Staphylococcus aureus
Flow cytometric analysis of ISgfp transposition and measurement of the transposition frequency of ISSau2. a E. coli strain harboring pET28a-ISgfp as a negative control. b E. coli strain harboring pET28a-ISgfp and pET32a-OrfAB. c E. coli strain harboring pET28a-ISgfp, pET32a-OrfAB and pET32a-OrfA. d E. coli strain harboring pET28a-ISgfp, pET32a-OrfAB and pET32a-OrfB. Experiments were performed in triplicates and representative results from one experiment are shown. The range of gated events is indicated as marker 1 (M1)