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Fig. 4 | Mobile DNA

Fig. 4

From: Characterization of a functional insertion sequence ISSau2 from Staphylococcus aureus

Fig. 4

In vitro excision of ISSau2. The artificial mini-transposon pUC19-IRL-gfp-IRR was incubated for 16 h in the absence or presence of OrfAB, OrfA or OrfB. Products were separated by agarose gel electrophoresis. SC, LN and OC represent supercoiled, linear and open circle pUC19-IRL-gfp-IRR, respectively; Vector stands for the vector backbone; ETF stands for the excised mini-transposon fragment

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