Fig. 3
From: Characterization of a functional insertion sequence ISSau2 from Staphylococcus aureus
Western blot analysis of ISSau2 and EMSA analysis of binding of OrfAB to inverted repeats. a Western blot analysis of the protein extracts from BL21 with pET28-ISSau2. Lane 1, protein extracts from BL21 with pET28-ISSau2; lane 2, protein extracts from BL21 with pET28b as a negative control; lane 3, protein extracts from BL21, as a blank control; lane 4, Protein Marker. b Western blot analysis of the protein extracts from BL21 with pET32-OrfAB. Lane 1, Protein Marker; lane 2, protein extracts from BL21 with pET32a as a negative control; lane 3, protein extracts from BL21 with pET32-OrfAB. c EMSA analysis of binding of OrfAB to left inverted repeat (IRL). Lane 1, biotin-labeled IRL only; lane 2, biotin-labeled IRL incubated with protein extracts from BL21 with pET32a as a negative control; lane 3, biotin-labeled IRL incubated with purified protein OrfAB (0.42 μg). d EMSA analysis of binding of OrfAB to right inverted repeat (IRR). Lane 1, biotin-labeled IRR incubated with purified protein OrfAB (0.42 μg); lane 2, biotin-labeled IRR incubated with protein extracts from BL21 with pET32a as a negative control; lane 3, biotin-labeled IRR only