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Fig. 1 | Mobile DNA

Fig. 1

From: Locus-specific hypomethylation of the mouse IAP retrotransposon is associated with transcription factor-binding sites

Fig. 1

Experimental design of TEPBAT. a Nucleotide sequence alignment of the consensus sequences of IAPLTR1, IAPLTR1a, IAPLTR2, IAPLTR2a2, and IAPLTR2b. The primer regions and orientations (IAP-BS1 and IAP-BS2) used in TEPBAT are indicated as green arrows. b Experimental design to enrich IAP regions in the bisulfite-treated genomic DNA. After bisulfite treatment, the first DNA strand was synthesized using the tag-plus-random primer, and the IAP regions were amplified by PCR using the specific primer (green arrow) and tag primer (red arrow)

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