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Fig. 3 | Mobile DNA

Fig. 3

From: Targeted identification of TE insertions in a Drosophila genome through hemi-specific PCR

Fig. 3

Read and primer support for true insertions and false positives detected by hemi-specific PCR. False-positives were detected by hemi-specific PCR but could not be validated by insertion-specific PCR or whole genome re-sequencing data, whereas true insertions were verified by one or both of these methods. a True insertions are sampled more sequencing libraries generated using different degenerate primers for hemi-specific PCR (Welch’s t 22 = 15.56, P = 2.91 × 10−13). b True insertions are supported by larger number of uniquely mapping read pairs in hemi-specific PCR libraries (Welch’s t 50 = 13.78, P < 2.2 × 10−16). The number of read pairs was normalized to reads per million based on total sequenced reads from each degenerate primer

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