Skip to main content
Fig. 7 | Mobile DNA

Fig. 7

From: Dynamic silencing of somatic L1 retrotransposon insertions reflects the developmental and cellular contexts of their genomic integration

Fig. 7

Lack of variegation and silencing of reporters newly mobilized by PB transposons. a, b Stable expression of TEM1 beta-lactamase reporter mobilized by PB DNA transposon in mouse ES (mES) cells. E14Tg2a.4 cells were transfected with PB vectors carrying the TEM-1 reporter as cargo. Upon staining with CCF2-AM substrate, resulting mES cells fluoresced either blue (stable expression of reporter) or green (no reporter), with minimal variegation observed. c, d Stable expression of TEM1 beta-lactamase reporter mobilized by PB in HeLa cells. No variegation was observed. Treatment with HDAC inhibitors did not increase the percentage of cells fluorescing blue (not shown), indicating a lack of silencing of reporter insertions that had been mobilized by PB

Back to article page