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Fig. 4 | Mobile DNA

Fig. 4

From: Dynamic silencing of somatic L1 retrotransposon insertions reflects the developmental and cellular contexts of their genomic integration

Fig. 4

Variable L1 reporter expression in cultured cancer cells is associated with changes in histone acetylation. Cultured human cervical cancer (HeLa) cells harboring de novo L1 reporter integrants were assayed for reporter beta-lactamase expression by incubating them with the fluorescent substrate, CCF2-AM. Left: Before and right: after incubation for 24 h with various histone deacetylase inhibitors including: a 10 mM butyrate; b 1 nM apicidin; and c 100 nM TsA. Similar responses also were observed upon treatment for 24 h with 1 uM scriptaid and with 5 mM nicotinamide respectively (Additional file 1: Figure S6)

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ISSN: 1759-8753