Fig. 8

Reversal of ORF orientation after self-insertion. a Helitron X (circle) inserts into an other (non-autonomous) Helitron Y in opposite orientation. The green bar indicates the ORF, striped sections indicate the inverted repeats, yellow line the 3’ terminus. b The composite HelitronXY. c Deletion or mutation of internal termini yields a Helitron with a ORF in opposite orientation when compared to other Helitrons with similar termini