Fig. 3

a Alignment of insertion sites confirms FoHeli termini. For each subgroup, we reconstruct pre-insertion sites by concatenating FoHeli flanking sequences and search for these pre-insertion sequences in our set of FOSC genomes. Alignment of FoHeli flanking sequenes with these pre-insertion sites showed that the termini we had inferred before are correct. Fom001 – F. oxysporum f.sp. melonis 26406, FoMN25 - F. oxysporum f.sp. lycopersici MN25, FoHDV247 - F. oxysporum f.sp. pisi HDV247, FoCL57 - F. oxysporum f.sp. radicis-lycopersici CL57, FoPHW815 - F. oxysporum f.sp. raphani PHW815, FoPHW808 - Fusarium oxysporum f.sp. conglutinans PHW808. In this example a FoHeli4 is inserted 2 bp from another FoHeli4 (indicated with * above the sequence). b Because FoHelis have a ‘T’ at the 5’ terminus and preferentially insert between ‘T’ and ‘A’, we can not be certain that e.g. FoHeli1s and FoHeli2s start with ‘TC’ like canonical Helitrons, or with ‘TTC’.