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Fig. 2 | Mobile DNA

Fig. 2

From: Non-canonical Helitrons in Fusarium oxysporum

Fig. 2

Terminal features and coding capacity for FOSC and other Helitrons. a FoHeli termini are characterized by hairpins and inverted repeats, where the 3’ inverted repeats is ~20–40 bp upstream from the terminal sequence. Within each of the two groups, termini are very similar. FoHeli1 and FoHeli2 have two hairpins, one at each terminus, a 12 bp long inverted repeat, start with ‘TCAG’ and end with ‘ATTTT’. Similar to canonical Helitrons, the 3’ inverted repeat and hairpin are located at ~30-40 bp from the 3’ terminus. In the other group, all FoHelis start with ‘TGCCT’ and end with ‘CTCCTGT’. At the 5’ end, they have a hairpin but they lack a hairpin at the 3’ end. The ORFs in FoHeli4 and FoHeli5 have an opposite orientation when compared to FoHeli3. FoHeli1 and FoHeli2 insert between ‘TNAT’ and ‘T’, for the other group we could not establish an insertion preference. b When we compare these structural features to those of known Helitrons, we find that FOSC Helitrons resemble Helitron2 transposons. Structural features of Helitron1 (canonical Helitrons), Helitron2 and Helentrons were compiled from [2, 7] and RepBase [51]. Helitron1/canonical Helitrons insert between ‘A’ and ‘T’, Helitron2 between ‘TTTT’ and ‘T’ or ‘C’ and Helentrons in a ‘TT’ dinucleotide. See Additional file 1: Figures S13 and S14 for more detail on Helitron domain composition

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