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Fig. 5 | Mobile DNA

Fig. 5

From: Deciphering fact from artifact when using reporter assays to investigate the roles of host factors on L1 retrotransposition

Fig. 5

MKK3b2E and pcDNA-MKK62E increase Rluc luminescence. a Mean Fluc (left) and Rluc (right) luminescence values obtained from lysates of HeLa cells transfected with the L1 reporter plasmid pYX015 or pYX017 in the presence of pcDNA-MKK3b2E (M3) or pcDNA-MKK62E (M6). Averages were derived from data shown in (b) by first averaging technical replicates for each biological sample (n = 2), then using this value to average biological replicates; error bars represent SEM of biological samples, n = 3. b Individual luminescence values are shown for Fluc (blue) and Rluc (red) obtained from lysates transfected with pYX015 or pYX017 and the indicated pcDNA expression vectors; technical replicates are side-by-side; biological replicates are indicated with subscripts. c Wells show effects on cell growth in response to expression of pcDNA-MKK3b2E (M3) or pcDNA-MKK62E (M6)

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