Distribution of the DNA transposon family, Pokey in the Daphnia pulex species complex

Table 3 Haploid number of Pokey, mPok, and rRNA genes in four isolates of North American Daphnia pulicaria

	PC1.1	PC1.2	PC2.1	PC2.2
gene^a	Average 1^b	High 1	High 2	Average 2
18S	283	460	176	385.5
28S	358.5	576.5	234.5	474.5
rPokeyA	6	17	48	3.5
gPokeyA	3.5	7.5	0	5
gPokeyB	5	6.5	9	6
gmPok2	1	0	0.5	0.5
mean number of differences from consensus^c	49.9	10.8	1.6	45.8
standard deviation	43.3	36.9	3.4	32.5
range	0 to 97	0 to 128^d	0 to 6	12 to 78
p-value^e		0.027		0.010

^a18S = 18S rRNA genes. 28S = 28S rRNA genes. rPokeyB, rmPok2 and mPok1 were not detected in these four isolates
^bAverage and High correspond to the sequence labels in Fig. 4
^cThe number of differences between the consensus and each of 12 cloned 1500 bp sequences was determined for each isolate. Gaps were included in the analysis. Consecutive gaps were counted as one variant nucleotide position
^dAll other values were 0 or 1 for this isolate
^eANOVA was used to compare the mean number of differences across all four isolates. This test was significant (F = 6.62, d.f. = 3, p = 0.0009). A Post hoc Tukey's HSD test was used to determine if means in the Average and High isolates from the same population were significantly different. They p-values refer to the Tukey’s tests

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