Skip to main content
Fig. 4 | Mobile DNA

Fig. 4

From: The endonuclease domain of the LINE-1 ORF2 protein can tolerate multiple mutations

Fig. 4

Acute toxicity assay in HeLa and 293 cells transiently transfected with ORF2 putative phosphorylation mutant plasmids. a HeLa cells were cotransfected with a NeoR expression vector and the indicated ORF2 putative phosphorylation mutant plasmid. b 293 cells were cotransfected with a NeoR expression vector and the indicated ORF2 putative phosphorylation mutant plasmid. In both panel a and b ORF2 is the functional protein and ORF2 EN-RT- is a non-functional protein containing mutations in the endonuclease (D205A) and reverse transcriptase (D702A) domains. Control indicates cells transfected with an empty vector and the NeoR expression vector. Colony formation was assayed after 2 weeks under G418 selection (Y-axis) and used as a measure of toxicity as previously described [26, 42]

Back to article page