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Fig. 2 | Mobile DNA

Fig. 2

From: The endonuclease domain of the LINE-1 ORF2 protein can tolerate multiple mutations

Fig. 2

Schematic representation of the putative phosphorylation sites within ORF2 mutated in this study. Numbered arrows indicate the locations of the putative phosphorylation sites mutated in this study. The boundaries of the ORF2 endonuclease domain are indicated with green brackets. Plasmids encoding for the full-length ORF2p were generated with the following mutations: S29A; S33A; S37A; S79A; S188A; S228A; S29A/S37A; S79A/T82A; S188A/T189A; S29A/S37A/S228A; S29A/S37A/S188A/T189A; S312A; S335A; and S312A/S335A. ORF2 11m contains the following mutations (red ovals): S29A/S33A/S37A/S151A/S188A/T189A/T220A/T224A/S228A/S312A/S335A. Plasmids encoding for the endonuclease domain (ENp) alone were generated with the following mutations: S29A; S33A; S37A; S79A; S188A; S228A; S29A/S37A; S79A/T82A; S188A/T189A; S29A/S37A/S228A; and S29A/S37A/S188A/T189A. EN 9m contains the following mutations (blue boxes): S29A/S33A/S37A/S151A/S188A/T189A/T220A/T224A/S228A. Plasmids encoding for the full-length L1 containing the following mutations within ORF2 were generated: S29A; S33A; S312A; S335A; and S312A/S335A

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