Fig. 10
From: The endonuclease domain of the LINE-1 ORF2 protein can tolerate multiple mutations
Retrotransposition driven by full-length L1 elements containing mutations in putative phosphorylation sites within ORF2. a Alu retrotransposition: Full-length L1 elements containing the indicated putative phosphorylation mutations within the ORF2 sequence were used to drive Alu retrotransposition in HeLa cells, as previously described [3]. L1 is the functional element and L1 EN- is a non-functional element containing a mutation in the ORF2 endonuclease domain (D205A). Control indicates cells transfected with an empty vector and the Alu retrotransposition reporter plasmid. The graph depicts the relative number of Alu retrotransposition events as represented by NeoR colonies (Y-axis). b L1 retrotransposition: Full-length L1 elements containing the indicated putative phosphorylation mutations within the ORF2 sequence were used in an L1 retrotransposition assay in HeLa cells, as previously described [5, 56]. L1 is the functional element and control indicates cells transfected with an empty plasmid. The graph depicts the relative number of L1 retrotransposition events as represented by NeoR colonies (Y-axis)