Table 1 The orientation of AT insertions identified by iPCR and sequencing as reported by Genome ARTIST

←^r←^b or ^b→^r→

Type I orientation. When both arrows are pointing to the same sense and the red arrow (←^r or ^r→) is the closest one relative to an imaginary target point, it means that the junction was sequenced at the 5′ end of the transposon. The variant on the left stands for sequencing with a primer facing towards the 5′ TIR (such as Sp1 for P{lacW}) and the variant on the right stands for sequencing with a primer facing towards the restriction site sequence (such as Plac4 for P{lacW}).

^r→^b→ or ←^b←^r

Type I orientation. When both arrows are pointing to the same sense and the blue arrow (←^b or ^b→) is the closest one relative to an imaginary target point, it means that the junction was sequenced at the 3′ end of the transposon. The variant on the left stands for sequencing with a primer facing towards the 3′ TIR (such as Spep1 for P{lacW}) and the variant on the right stands for sequencing with a primer facing towards the restriction site sequence (such as Sp6 for P{lacW}).

←^{r b}→ or ←^{b r}→

Type II orientation. When the blue and red arrows are pointing away from each other (a divergence of the senses), it means that the junction was sequenced at the 5′ end of the transposon. The variant on the left stands for sequencing with a primer facing towards the 5′ TIR (such as Sp1 for P{lacW}) and the variant on the right stands for sequencing with a primer facing towards the restriction site sequence (such as Plac4 for P{lacW}).

^r→←^b or ^b→←^r

Type II orientation. When the blue and red arrows are pointing to each other (a convergence of the senses), it means that the junction was sequenced at the 3′ end of the transposon. The variant on the left stands for sequencing with a primer facing towards the 3′ TIR (such as Spep1 for P{lacW}) and the variant on the right stands for sequencing with a primer facing towards the restriction site sequence (such as Sp6 for P{lacW}).