Fig. 5

Simulation of small-scale mutations affecting nucleotides located close to the TIR. The mutations were modeled in a region equivalent to TSD, which is represented herein by the arbitrary octet CCAAACTT (blue). With reddish are highlighted the partial sequences of the two TIRs specific for P{lacW} construct. TGN I (a T nucleotide) and TGN II (a C nucleotide) are capitalized inside the respective TSD boxes. The nucleotides affected by simulations in TSD are those located in the relative positions 2, 3, 4, 5 and 6 as sliding away from each TGN toward the other end of TSD. The drawing was realized with CLC Main Workbench software v.6.9 (CLC Bio-Qiagen, Aarhus, Denmark)