Fig. 1

Phylogeny of sampled Oxytricha TBE genes and orthologs identified in three other stichotrich ciliates. a Schematic map of TBE transposons. Gray arrows represent terminal inverted repeats (TIR). Orange arrows represent ORFs encoded by TBEs. b Phylogeny constructed with TBE 42kD transposases (29 TBE1, 27 TBE2.1, 26 TBE2.2 and 25 TBE3 42kD protein sequences). Clades formed by TBE1, TBE2 and TBE3 are labeled accordingly. TBE2.1 representatives are indicated in red and TBE2.2 in blue. Internal branches supported by posterior probability higher than 0.9 are colored in green. c Phylogeny constructed with TBE 22kD ORFs (32 TBE1, 39 TBE2.1, 30 TBE2.2 and 28 TBE3 22kD protein sequences). Colors are as above. d Phylogeny constructed with TBE 57kD ORFs (27 TBE1, 26 TBE2.1, 23 TBE2.2 and 21 TBE3 57kD protein sequences). Clades formed by TBE1, TBE2.1, TBE2.2 and TBE3 are labeled accordingly; colors as above. The multiple sequence alignment was produced with MAFFT v6.956b and trimmed with trimAl v1.2 to remove excess gaps and poorly aligned regions. The unrooted Bayesian trees were produced with MrBayes v3.2.2 [35]. The three TBE orthologs are 1: Sterkiella histriomuscorum; 2: Tetmemena sp.; 3: Laurentiella sp.. All posterior probability values are above 0.5. The scale below the phylogeny illustrates branch substitutions per site