Ribosomal protein and biogenesis factors affect multiple steps during movement of the Saccharomyces cerevisiae Ty1 retrotransposon

Table 1 Quantitative Ty1 mobility is reduced in ribosomal protein gene Ty1 cofactor mutants

Strain	Function^a	Ty1 mobility ± SEM (× 10⁻⁷)^b	Fold reduced from WT
WT	–	40 ± 2.0	–
rpl1B∆	LSU protein	4.7 ± 1.1	8.5
rpl4A∆	“	1.9 ± 0.56	21
rpl15B∆	“	2.6 ± 0.78	16
rpl21A∆	“	4.9 ± 1.3	8.1
rpl27A∆	“	6.5 ± 1.6	6.2
rpl39∆	“	0.19 ± 0.14	210
rpl41B∆	“	8.1 ± 1.9	4.9
rpp2B∆	“	3.0 ± 1.0	13
rps0B∆	SSU protein	5.6 ± 0.81	7.2
rps9B∆	“	4.7 ± 0.79	8.5
rps10A∆	“	4.8 ± 0.66	8.3
rps19B∆	“	1.4 ± 0.27	29
rps25A∆	“	7.8 ± 1.4	5.1
rrp6∆	LSU processing	2.8 ± 0.56	14
rrp8∆	SSU processing	4.0 ± 0.79	10
kap123∆	60S nuclear export	<0.54^c	>74
bem4∆	Budding	<0.11^c	>340
spe3∆	Polyamine synthesis	0.67 ± 0.67	59

^aLSU = large (60S) ribosomal subunit; SSU = small (40S) ribosomal subunit
^bMobility was calculated from a minimum of five repeated experiments as number of His⁺ cells per number of total viable cells
^cNo observed mobility; the maximum mobility is less than assuming mobility calculated if one event had occurred in the number of assays performed

ISSN: 1759-8753