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Fig. 5 | Mobile DNA

Fig. 5

From: Ribosomal protein and biogenesis factors affect multiple steps during movement of the Saccharomyces cerevisiae Ty1 retrotransposon

Fig. 5

Ty1i RNA and Gag-p22/p18 expressed in ribosomal protein deletions. a Northern blot of total RNA from wild type (WT) and four mutant strains (spt3∆, rpl27A∆, rpl21A∆, rps0B∆ and rpl39). A 32P-labeled riboprobe of Ty1 GAG (nt 1266-1601) hybridized to with full-length Ty1 mRNA (“Ty1”) and, especially in the spt3∆ mutant strain, to the subgenomic inhibitory Ty1i mRNA (“Ty1i”). b A Northern blot of poly(A)+ mRNA from the same strains. The same probe hybridized to Ty1 and Ty1i mRNAs. c Total protein extracts from the same strains was immunoblotted with a p18-specific antiserum to detect p49/p45/Gag and p22/p18. The histidyl-tRNA synthetase (Hts1) served as a loading control

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