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Fig. 4 | Mobile DNA

Fig. 4

From: Ribosomal protein and biogenesis factors affect multiple steps during movement of the Saccharomyces cerevisiae Ty1 retrotransposon

Fig. 4

Subunit-specific changes in the steady-state level of Ty1 mRNA by cofactor mutants. Total cellular RNA was hybridized with an [32P]-labeled DNA probe specific to the mRNA encoding the RT domain of the Ty1 element (upper panel) or PYK1 control transcript (lower panel), which serves as a lane to lane control for the amount of RNA in the samples. The intensity of hybridization by the Ty1 probe relative to the PYK1 probe was quantified using the ImageJ software. The asterick (*) shows the position of the internally initiated Ty1i RNA detected in some of the mutants. LSU = large (60S) ribosomal subunit; SSU = small (40S) ribosomal subunit

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Mobile DNA

ISSN: 1759-8753