Table 1 Genes/Coding Sequences located 5′ and 3′ to BrickBuilt elements. Gene numbers and characterizations correspond to strain ATCC 33277. Loci of BrickBuilt elements across four sequenced and annotated P. gingivalis strains. BrickBuilt elements are situated intergenically between the genes noted. Grayed-out boxes represent loci at which BrickBuilt is aberrant
MITE | 33277 Locus | W83 Locus | TDC60 Locus | HG66 Locus | Gene Characterization | Gene | Strand |
|---|---|---|---|---|---|---|---|
BrickBuilt_1 | PGN_0031 | PG0033 | PGTDC60_0032 | EG14_02395 | RmuC domain (DUF 805) | Â | - |
| Â | PGN_0033 | PG0034 | PGTDC60_0034 | EG14_02400 | Thioredoxin | trx | - |
BrickBuilt_2 | PGN_0204 | PG2159 | PGTDC60_1262 | EG14_03005 | Protoporphyrinogen oxidase | hemG | + |
| Â | PGN_0205 | PG2161 | PGTDC60_1265 | EG14_03010 | AraC family transcriptional regulator | Â | - |
BrickBuilt_3 | PGN_0303 | PG0196 | PGTDC60_0466 | EG14_04795 | Zinc protease (Peptidase M16) | Â | + |
| Â | PGN_0306 | PG0198 | PGTDC60_0471 | EG14_04805 | PF05656 family protein (DUF 805) | Â | + |
BrickBuilt_4 | PGN_0336 | NP | PGTDC60_1661 | EG14_04940 | Immunoreactive antigen/PorSS CTD | Â | - |
| Â | PGN_0340 | NP | PGTDC60_1665 | EG14_04960 | Peptidase S41/PorSS CTD | Â | + |
BrickBuilt_5 | PGN_0361 | PG0264 | PGTDC60_0543 | EG14_05065 | Glycosyl transferase family 2 | Â | + |
| Â | PGN_0365 | PG0267 | PGTDC60_0547 | EG14_05075 | Arginyl-tRNA synthetase | argS | + |
BrickBuilt_6 | PGN_0400 | PG1715 | PGTDC60_0586 | EG14_05255 | TonB-dependent receptor Cna protein | Â | + |
| Â | PGN_0403 | PG1714 | PGTDC60_0590 | EG14_05265 | Pyridoxamine-phosphate oxidase | pdxH | + |
BrickBuilt_7 | PGN_0455 | PG0549 | PGTDC60_0639 | EG14_03135 | Partial ISPg5 | Â | + |
| Â | PGN_0456 | PG0553 | PGTDC60_0641 | EG14_03150 | Methylmalonyl-CoA mutase | scpA | - |
BrickBuilt_8 | PGN_0550 | PG1559 | PGTDC60_0739 | EG14_03610 | Glycine cleavage system subunit T | gcvT | + |
| Â | PGN_0553 | PG1556 | PGTDC60_0743 | EG14_03615 | Conserved hypothetical (DUF2149) | Â | - |
BrickBuilt_9 | PGN_0558 | PG1548 | PGTDC60_0748 | EG14_03640 | Haem-binding protein | hmuY | - |
| Â | PGN_0559 | PG1550 | PGTDC60_0751 | EG14_03655 | Serine protease (Peptidase C10) | prtT | - |
BrickBuilt_10 | PGN_0632 | PG0585 | PGTDC60_1709 | EG14_09225 | Aspartyl-tRNA amidotransferase B | Â | + |
| Â | PGN_0633 | PG0587 | PGTDC60_1713 | EG14_09235 | Membrane protein putative ion channel | btuF | - |
BrickBuilt_11 | PGN_0667 | PG0625 | PGTDC60_1753 | EG14_09060 | GTP cyclohydrolase I/PorSS CTD | folE | + |
| Â | PGN_0668 | PG0627 | PGTDC60_1756 | EG14_09045 | RNA-binding protein/PorSS CTD | Â | - |
BrickBuilt_12 | PGN_0819 | PG0796 | PGTDC60_1912 | EG14_08255 | Leucyl-tRNA synthetase | leuS | - |
| Â | PGN_0823 | PG0800 | PGTDC60_1917 | EG14_08240 | NAD-utilizing dehydrogenase | Â | - |
BrickBuilt_13 | PGN_0831 | PG0807 | PGTDC60_1926 | EG14_08205 | N utilization substance/PorSS CTD | Â | - |
| Â | PGN_0832 | PG0809 | PGTDC60_1927 | EG14_08200 | Gliding motility protein/PorSS CTD | sprA | - |
BrickBuilt_14 | PGN_0871 | PG1389 | PGTDC60_2074 | EG14_07995 | Membrane protein | Â | - |
| Â | PGN_0872 | PG1391 | PGTDC60_2073 | EG14_08000 | DNA-binding protein (PF00216) | Â | + |
BrickBuilt_15 | PGN_0898 | PG1424 | PGTDC60_2039 | EG14_07870 | Peptidylarginine deiminase/PorSS CTD | PAD | - |
| Â | PGN_0900 | PG1427 | PGTDC60_2036 | EG14_07865 | Peptidase C10/PorSS CTD | Â | - |
BrickBuilt_16 | PGN_1207 | PG1117 | PGTDC60_1098 | EG14_06320 | Transport multidrug efflux | Â | + |
| Â | PGN_1208 | PG1118 | PGTDC60_1096 | EG14_06310 | ClpB chaperone and protease | clpB | - |
BrickBuilt_17 | PGN_1476 | PG0494 | PGTDC60_1611 | EG14_09640 | PorSS C-terminal sorting domain | Â | - |
| Â | PGN_1479 | PG0491 | PGTDC60_1606 | EG14_09650 | Peptidase S10/PorSS CTD | dppVII | - |
BrickBuilt_18 | PGN_1777 | PG1784 | PGTDC60_0106 | EG14_00235 | Cysteine protease (Peptidase C1) | Â | - |
| Â | PGN_1780 | PG1786 | PGTDC60_0110 | EG14_00245 | Endoribonuclease L-PSP | Â | - |
BrickBuilt_19 | PGN_2035 | PG0088 | PGTDC60_0367 | EG14_01925 | Peptidase M16 | Â | + |
| Â | PGN_2037 | PG0090 | PGTDC60_0370 | EG14_01930 | DNA-binding protein from starved cells | dps | + |