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Fig. 3 | Mobile DNA

Fig. 3

From: A host factor supports retrotransposition of the TRE5-A population in Dictyostelium cells by suppressing an Argonaute protein

Fig. 3

CbfA controls AgnC expression. a Scheme of the CbfA protein. The 1000-amino acid protein can be roughly divided into an amino-terminal part that may have chromatin-remodeling activity and a carboxy-terminal part that may facilitate DNA-binding [28, 39]. JmjC: carboxy-terminal jumonji domain", ZF: zinc finger-like motif; NRD: asparagine-rich domain; CTD: carboxy-terminal domain. The CbfA proteins expressed in mutant JH.D comprised either the full length protein (amino acids 2–1000) or the CbfA-CTD (amino acids 724–1000). b Expression of CbfA in JH.D cells. CbfA mutant JH.D cells were transformed with plasmids allowing for the expression of either untagged, full-length CbfA [21] or the GFP-tagged, carboxy-terminal domain of CbfA (CbfA-CTD) [28]. Shown is a western blot of whole-cell extracts prepared from the indicated strains. CbfA was visualized with the monoclonal antibody 7 F3 that detects CbfA-CTD. Numbers to the left indicate the sizes of the protein standards in kDa. c Complementation of the agnC overexpression phenotype in JH.D cells. Expression of agnC in the indicated strains was determined by qRT-PCR. Expression levels in JH.D cells and JH.D transformants were compared to AX2 wild-type cells and are expressed as “fold change” of expression, meaning that values >1 represent overexpression of genes in the JH.D strains and a value of 1 would indicate complete reversion of the overexpression in JH.D cells. Values are means from six independent cultures ± SD. **p < 0.01, relative to control AX2 cells (Student’s t-test)

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