From: A droplet digital PCR detection method for rare L1 insertions in tumors
Primer/Probe Name | Sequence |
---|---|
5’ L1Hs Primer | 5’ GGAAATGCAGAAATCACCGTCTTC 3’ |
5’ L1Hs Probe (Chr 15) | 5’ FAM AGGAACAGCTCCGGTCTACAGCTC BHQ1 3’ |
5’ L1Hs Probe (other loci tested) | 5’ FAM AGGAACAGC/ZEN/TCCGGTCTACAGCTC IABkFQ 3’ |
5’ Chr15 AC216176 Locus Primer | 5’ GTGGACAAAGAAAAGCATCCTTGAT 3’ |
RPP30 Forward Primer | 5’ GATTTGGACCTGCGAGCG 3’ |
RPP30 Reverse Primer | 5’ GCGGCTGTCTCCACAAGT 3’ |
RPP30 Probe | 5’ VIC CTGACCTGAAGGCTCT MGB 3’ |
5’ Chr4 esv3475 Locus Primer | 5’ CCACATGGTATAAGATAAAAACACGAG 3’ |
5’ Chr4 esv4912 Locus Primer | 5’ CTAAGCAATGGAGGAAAATATCG 3’ |
3’ L1Hs Primer | 5’ GGGAGATATACCTAATGCTAGATGACAC 3’ |
3’ L1Hs Probe | 5’ FAM ATTATACTCTAAGTTTTAGGGTACATGTGCACATTGTGC BHQ1 3’ |
3’ Chr15 AC216176 Locus Primer | 5’ TCTATAAGCAGTGGAAGCACATG 3’ |
3’ Chr4 esv3475 Locus Primer | 5’ GACCAATTTTTTTTTGCCTGTACTGAC 3’ |
3’ Chr4 esv4912 Locus Primer | 5’ TGCATTTTGAGTTAATTTTTGTACATGGTG 3’ |