Figure 1
From: A droplet digital PCR detection method for rare L1 insertions in tumors
Schematic of L1Hs droplet digital PCR (ddPCR) assays. (A) A 5’-FAM labeled Taqman™ probe specific to the 5’ UTR of L1Hs is paired with an L1 5’ UTR-specific primer. The probe and primer anneal to complementary DNA strands. This primer/probe set can be used in conjunction with a unique genomic flanking primer to detect the 5’-insertion junction of specific full-length L1 elements in the human genome using ddPCR. A control assay using a primer and 5’-VIC labeled probe set specific to a housekeeping gene (RPP30) can be used in parallel to determine copy number. (B) A 5’-FAM labeled Taqman™ probe specific to the 3’ end of L1Hs is paired with an L1Hs-specific primer. The probe and primer anneal to complementary DNA strands. This primer/probe set can be used in conjunction with a unique genomic flanking primer to detect the 3’ insertion junction of specific L1Hs elements in the human genome using ddPCR.