IS50 transposase mRNA half-life analysis. Strains TM338 (wt) and TM618 (hfq−) were transformed with IS50 transposase encoding plasmid pDH533 and total RNA was isolated either before or after the addition of rifampicin (at the indicated time points). Transposase RNA was detected as described in Figure 5. The bands were quantified (ImageQuant) and T1 normalized to un-extended primer before plotting the proportion of RNA remaining after rifampicin addition (time zero = 1.0). The data was fit to a one-phase exponential decay curve by non-linear regression (Prism) to determine the half-life (t1/2). The data shown is a compilation from two independent experiments.