Figure 4From: Homing endonucleases from mobile group I introns: discovery to genome engineering Redesign of a LAGLIDADG homing endonuclease (HE; also termed a ‘meganuclease’) for a specific genome engineering application (such as modification of a disease-associated human gene locus) involves the alteration of a substantial fraction of its DNA-contacting residues, as well as further optimization of neighboring positions on the protein scaffold. Back to article page