Figure 3

Schematic representations of intein-mediated post-translational processing. In all panels, POI indicates protein of interest. A and B. Intein-mediated cyclization. In A, 'N’ indicates either an intein that generates an N-terminal Cys on the POI by C-terminal cleavage or a protein or peptide removed by proteolysis to reveal an N-terminal Cys. C. Intein-mediated protein cyclization. D. Generation of a protein with a uniform N-terminal residue. E. Post-translational peptide generation. F. Generation of antibodies by a single open reading frame (ORF). HC indicates heavy chain and LC indicates light chain. G. Use of an intein as a highly specific protease. H. Selection for internal GFP labeling.