Figure 2
From: Molecular characterization of a new member of the lariat capping twin-ribozyme introns
Proposed base-pairing interactions between the LC ribozyme and HEG. (A) Schematic box presentation of pHEG-1, pHEG-2 and AP2.1 in Allovahlkampfia and pHEG-1, pHEG-2, pHEG-3, and NP2.1 in N. pringsheimi twin-ribozyme introns, respectively. P6 is from the GIR2 ribozyme. 5′ (n′) and 3′ (n″) sequence boxes are indicated, as well as the start (AUG) and stop (UGA) codons of the HEG. (B) Secondary structure diagram of the LC-GIR2 junctions in Asp.S516 and Npr.S516. Corresponding HEGs (red letters) are indicated by start codon (AUG), polyA signal (AAUAAA), and stop codon (UGA). pHEG-1, pHEG-2 and P2.1 are present in both introns and indicate base pairings between the LC ribozyme and HEG. AspGIR2 and NprGIR2, group I splicing ribozymes; AspLC and NprLC, lariat capping ribozymes; BP, branch point nucleotide; IPS, internal processing site.