Figure 1

Multiple sequence alignment based on protein sequences of the 64 analyzed CACTA transposases. Colored boxes indicate amino acids, gray boxes indicate residues with a GUIDANCE score below 0.804, and white boxes indicate gaps in the multiple sequence alignment (MSA). The plot below the MSA shows GUIDANCE scores for the corresponding position in the MSA. Columns with a score below 0.804 are indicated in light blue while columns with a score of 0.804 and above in dark blue. Positions relative to the MSA and corresponding GUIDANCE score are shown between the MSA and the plot. Highly conserved DDE transposase motifs as described in [22] are depicted on top. In the phylogenetic tree, colors indicate the host as shown in the legend. Major clades are depicted α to θ. Exon/intron boundaries are depicted as blue circles if their GUIDANCE score was above 0.804 and red otherwise. The number in the boundary indicates the boundary number on the corresponding transposase. Regions I to III are indicated by dashed lines and corresponding roman capitals. Positions of putative intron gain are depicted as described in the legend.