Figure 1

Properties of the family 2 casposons. (A) Predicted common protein-coding genes within family 2 casposons include a PolB family polymerase, an HNH family endonuclease, several HTH domains, and Cas1. The gene color code corresponds to that of Krupovic et al. The green arrows flanking the casposons indicate target site duplications (TSDs). (B) An alignment of the first 41 nucleotides (nt) of casposon family 2 Left End Terminal Inverted Repeats (TIRs) reveals conserved sequence motifs which could be the basis of transposase recognition. Green letters indicate the TSDs and black letters the TIR sequences identified by Krupovic et al., with apparently conserved patterns highlighted in red or blue. Bold black lettering corresponds to nts that were not included in the analysis of Krupovic et al. The aligned sequences and the Accession Number and coordinates for each are: MetFor-C1 [NC_019943;1964105..1964159], MetPsy-C1 [NC_018876;190336..190390], MetTin-C1 [NZ_AZAJ01000001; 3015399..3015453], MetMaz-C1 [NC_003901; 3946587..3946641], MetMah-C1[NC_014002; reverse complement of 1332841..1332895], MetLum-C1 [NZ_CAJE01000015; 159864..159918] AciBoo-C1 [NC_013926; 380309..380363], MetArv-C1 [NC_009464; 2695204..2695258].