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Figure 7 | Mobile DNA

Figure 7

From: Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis

Figure 7

Thermotargetron expression vector and DNA target site recognition. (A) The thermotargetron expression vector pHK-TTA1 carries replication origins for E. coli (ColE1 ori) and Gram-positive bacteria (repB ori) and a chloramphenicol-resistance marker gene (catR) that functions in both Gram-negative and Gram-positive bacteria. The thermotargetron cassette consisting of the upstream Thermosynechococcus elongatus TeI3c group IIB intron and the downstream TeI4c RT is expressed from a Clostridium thermocellum groEL promoter. (B) The figure shows DNA target site interactions for the wild-type TeI3c group IIB intron used in the thermotargetron. Portions of the intron RNA involved in EBS1-IBS1, EBS2-IBS2, and EBS3-IBS3 base-pairings interactions with the DNA target site are shown in red. Purple highlights indicate the two base-pairs in the 5’ exon (E1) that are important for DNA targeting and are likely recognized by the TeI4c reverse transcriptase (RT). IS, intron-insertion site.

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Mobile DNA

ISSN: 1759-8753