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Figure 4 | Mobile DNA

Figure 4

From: Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis

Figure 4

DNA target site recognition by mobile group II introns. The figure shows DNA target-site interactions for the Ll.LtrB group IIA intron and the EcI5 and RmInt1 group IIB introns used as targetrons. Portions of the intron RNA involved in the EBS1-IBS1, EBS2-IBS2, and δ − δ’ or EBS3-IBS3 base-pairing interactions with the DNA target site are shown in red. Purple and blue highlights indicate base-pairs in the 5’ and 3’ exons (E1 and E2, respectively) that are important for DNA targeting and recognized by the reverse transcriptase (RT). CS, bottom-strand cleavage site; IS, intron-insertion site.

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Mobile DNA

ISSN: 1759-8753