Impact of ploidy level on the distribution of Pokey element insertions in the Daphnia pulex complex

Table 3 Correlations between Pokey and rRNA gene number in diploid and polyploid Daphnia from North America

Cytotypes	X-axis	Y-axis	Slope	y-intercept	R²	P-value	Figure
All diploids	18S	28S	1.8038	−39.8411	0.9901	1.76 e-08*	AF6⁸
All triploids	18S	28S	1.6627	−14.3154	0.9586	5.07 e-07*	AF6
Diploid hybrids	Hpl ¹	Pokey (TED)²	16.500	11.674	0.0634	0.4549	2
	18S	rPokey³	−0.0146	9.4540	0.1035	0.4371	AF7
	gPokey⁴	rPokey	−06251	13.0792	0.3927	0.0964	AF8
	Pokey-1 (TED)⁵	Total gPokey⁶	1.5329	−1.7681	0.4950	0.0515	3
Diploid hybrids - PX2-MB-1	18S	rPokey	0.0147	−1.07571	0.3979	0.1287	AF7
Diploid hybrids - PX2-MB-1	gPokey	rPokey	−0.3350	8.0216	0.5605	0.0528	AF8
Diploid hybrids – (PX2-QC-9, PX2-MI-7)	Pokey-1 (TED)	Total gPokey	1.5741	−2.7883	0.7383	0.0283*	-
	Pokey-1 (TED)	H _Pokey ⁷	−0.0088	0.7623	0.0376	0.7130	AF9
	Hpl	H _Pokey	−3.2220	1.8820	0.6592	0.0497*	AF10
Triploid hybrids	Hpl	Pokey (TED)	6.6490	17.534	0.0756	0.4418	2
	18S	rPokey	0.0085	1.2673	0.2492	0.2541	AF7
	gPokey	rPokey	0.0201	2.8478	0.0036	0.8978	AF8
	Pokey-1 (TED)	Total gPokey	−1.6300	1.0290	0.3342	0.1740	3
Triploid tenebrosa	Pokey-1 (TED)	Total gPokey	−0.1923	20.7692	0.4808	0.5122	3

1. Denotes average heterozygosity estimated using nine microsatellite loci weighted by the ploidy level.
2. Denotes Pokey insertion site number estimated using TE display.
3. Denotes haploid Pokey number in 28S genes amplified using qPCR.
4. Denotes haploid Pokey number outside 28S genes amplified using qPCR.
5. Denotes Pokey insertion site number estimated using TE display minus the peak from elements in 28S genes.
6. Denotes total Pokey number outside 28S genes amplified using qPCR.
7. Denotes average heterozygosity of Pokey insertions outside 28S genes amplified using qPCR.
8. AF refers to Additional Files.
*P values are significant results.

ISSN: 1759-8753