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Table 2 Summary of TE display and qPCR analyses of Pokey number in diploid and polyploid isolates in the Daphnia pulex complex

From: Impact of ploidy level on the distribution of Pokey element insertions in the Daphnia pulex complex

Isolates TE display qPCR
Anneal at 50°C Anneal at 55°C 18S genes 28S genes Total Pokey rDNA Pokey Genome Pokey Tif:Gtp
(Total-rDNA)
Diploids with known hybrid status 19.09 ± 3.99 [11] 13.83 ± 4.22 [6] 293.25 ± 111.19 [8] 486.25 ± 201.36 [8] 17.81 ± 4.35 [8] 5.19 ± 5.03 [8] 12.63 ± 5.04 [8] 0.91 ± 0.06 [8]
Polyploids with known hybrid status 21.50 ± 3.54 [10] 16.33 ± 1.75 [6] 214.57 ± 62.34 [7] 346.67 ± 113.86 [7] 15.57 ± 3.43 [7] 3.10 ± 1.07 [7] 12.48 ± 3.20 [7] 0.92 ± 0.07 [7]
Total diploids 16.64 ± 4.94 [14] 12.71 ± 4.86 [7] 292.78 ± 104.02 [9] 488.28 ± 188.45 [9] 16.67 ± 5.32 [9] 5.05 ± 4.72 [9] 11.61 ± 5.61 [9] 0.90 ± 0.06 [9]
Total polyploids 19.00 ± 4.36 [13] 14.44 ± 3.17 [9] 248.03 ± 75.21 [10] 398.10 ± 127.42 [10] 14.33 ± 3.45 [10] 3.77 ± 1.40 [10] 10.57 ± 4.03 [10] 0.92 ± 0.08 [10]
  1. Results from qPCR are average ± standard deviation of Pokey inserts per haploid genome whereas results from TE display are for whole genomes. Numbers in brackets are the number of isolates tested. Tif:Gtp ratio is the number of Tif relative to the number of Gtp single copy reference genes.