Table 2 Summary of TE display and qPCR analyses of Pokey number in diploid and polyploid isolates in the Daphnia pulex complex
From: Impact of ploidy level on the distribution of Pokey element insertions in the Daphnia pulex complex
Isolates | TE display | qPCR | ||||||
|---|---|---|---|---|---|---|---|---|
Anneal at 50°C | Anneal at 55°C | 18S genes | 28S genes | Total Pokey | rDNA Pokey | Genome Pokey | Tif:Gtp | |
(Total-rDNA) | ||||||||
Diploids with known hybrid status | 19.09 ± 3.99 [11] | 13.83 ± 4.22 [6] | 293.25 ± 111.19 [8] | 486.25 ± 201.36 [8] | 17.81 ± 4.35 [8] | 5.19 ± 5.03 [8] | 12.63 ± 5.04 [8] | 0.91 ± 0.06 [8] |
Polyploids with known hybrid status | 21.50 ± 3.54 [10] | 16.33 ± 1.75 [6] | 214.57 ± 62.34 [7] | 346.67 ± 113.86 [7] | 15.57 ± 3.43 [7] | 3.10 ± 1.07 [7] | 12.48 ± 3.20 [7] | 0.92 ± 0.07 [7] |
Total diploids | 16.64 ± 4.94 [14] | 12.71 ± 4.86 [7] | 292.78 ± 104.02 [9] | 488.28 ± 188.45 [9] | 16.67 ± 5.32 [9] | 5.05 ± 4.72 [9] | 11.61 ± 5.61 [9] | 0.90 ± 0.06 [9] |
Total polyploids | 19.00 ± 4.36 [13] | 14.44 ± 3.17 [9] | 248.03 ± 75.21 [10] | 398.10 ± 127.42 [10] | 14.33 ± 3.45 [10] | 3.77 ± 1.40 [10] | 10.57 ± 4.03 [10] | 0.92 ± 0.08 [10] |