The chromovirus Beon 1 of the Galadriel clade is located exclusively within the 18S rRNA (ribosomal RNA) genes. Members of the Beon 1 family were found to be integrated at a conserved position of the 18S rRNA gene throughout species of different Beta sections; the integration in the opposite direction to the analyzed Beon 1 copy within the Beta vulgaris bacterial artificial chromosome (BAC) is shown at the top. Beon 1 integration sites were amplified from the following species of the section Beta (B. vulgaris ssp. vulgaris KWS2320 (cultivated beet) and B. vulgaris ssp. maritima (wild beet)), and wild beets of the section Corollinae (B. lomatogona and B. macrorhiza). For the wild beet species B. vulgaris ssp. maritima, integration in the direction of rDNA transcription was confirmed by PCR. The wild beets B. lomatogona and B. macrorhiza harbor Beon 1 copies, which were found to have an integration pattern in both orientations. The conserved target site duplication of Beon 1 elements is indicated by grey boxes.