Figure 5

Genomic organization and abundance of the chromoviral clades CRM, Tekay, Reina, and Galadriel in species of the genera Beta and Patellifolia . Genomic Hin dIII-restricted DNA was analyzed by comparative Southern hybridization using a probe from the reverse transcriptase (RT) sequence of (A) Beetle 3 (CRM clade), (B) Bongo 3 (Tekay clade), (C) Bingo 1 (Reina clade), and (D) Beon 1 (Galadriel clade). The following species from the section Beta were analyzed: (1) B. vulgaris ssp. vulgaris KWS2320 (cultivated beet), (2) B. patula (wild beet). Wild beets were used from the sections Corollinae ((3) B. corolliflora), and Nanae ((4) B. nana). Additionally, he species Patellifolia procumbens (5) from the genus Patellifolia, formerly classified as Beta procumbens of the genus Beta was analyzed. Spinacia oleracea (6) was used as outgroup species. The exposition times were 8 hours for filters A and B, and 24 hours for filters C and D.