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Figure 1 | Mobile DNA

Figure 1

From: Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons

Figure 1

H2A and H2A.Z are selectively enriched with long terminal repeat (LTR) and long interspersed nuclear element-1 (LINE - 1) elements, respectively. (A) Schematic representations of full-length LINE-1, LTR (M mERV and IAP) and SINE-B1 elements. The position of the primer binding sites is indicated in arrows. (B) Chromatin immunoprecipitation (ChIP) was performed with anti-H2A, anti-H2A.Z or anti-AcH2A.Z affinity purified antibodies on chromatin preparations harvested from L929 and NIH3T3 cells. IgG represents non-specific background after immunoprecipitation. Genomic DNA was extracted and quantified by qPCR using consensus primers specific for the three classes of L1, LTR (intracisternal A-particle (IAP) and mouse endogenous retrovirus (Mm ERV)) and B1 retrotransposons. Enrichment was determined by 100 x 2^ (ΔCTinput - ΔCTenriched) after adjusting the percentage of input DNA used. Shown are average ± s.d. of three biological replicates (n).

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