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Figure 9 | Mobile DNA

Figure 9

From: PGBD5: a neural-specific intron-containing piggyBac transposase domesticated over 500 million years ago and conserved from cephalochordates to humans

Figure 9

PGBD5 partition in mouse brain. Whole frozen mouse brains were thawed, Dounced with a tight pestle, and partitioned as described in Methods. Crude nuclei were pelleted through a sucrose cushion to remove debris and residual cytoplasm. Aliquots corresponding to equal cell equivalents were assayed by western blotting for PGBD5, glutathione-S-transferase (cytoplasmic marker), and histone H3 (nuclear marker). Nuclear integrity was determined visually by DAPI staining. As in Figure 8, PGBD5 partitioned about equally between nucleus and cytoplasm.

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Mobile DNA

ISSN: 1759-8753