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Figure 8 | Mobile DNA

Figure 8

From: PGBD5: a neural-specific intron-containing piggyBac transposase domesticated over 500 million years ago and conserved from cephalochordates to humans

Figure 8

Immunoprecipitation of endogenous PGBD5 from mouse brain. Mouse whole brain homogenate (5 or 25 μL) was immunoprecipitated with anti-rPGBD5 antibody. The proteins were resolved by SDS-PAGE, blotted, and probed with the same anti-rPGBD5 antibody as in Figure 7. rPGBD5, recombinant PGBD5; E1 and E2, first and second SDS elutions from the beads. Note that proteins larger than PGBD5 are present in whole brain homogenate (rightmost lane) but do not immunoprecipitate (lanes E1 and E2).

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