Figure 5

PGBD5 expression in the adult and developing mouse brain. Panels A-D illustrate expression of PGBD5 in sagittal brain sections (right) and a corresponding view from the Allen Institute Brain Atlas (left). The age of the mouse and the sagittal position relative to midline (0.00 mm) are indicated within each panel. The in situ hybridization stains are from public expression databases including The Allen Institute for Brain Science [39] for the adult mouse and Max Planck’s Genepaint [38] for embryos. The atlas images are color coded: olfactory bulb (green); cerebellum (orange); medial pallium (red); hypothalamus (brown); prepontine hindbrain (purple); and medullary hindbrain (blue). PGBD5 expression is restricted to a subset of cells within each nucleus denoted by a more saturated color. For example, PGBD5 expression in the cerebellar granule cell layer is indicated by a darker orange than the surrounding cerebellar cortex. The Max Planck and Allen Institute in situ hybridization probes both span exons 2–7, and are nearly identical; the absence of exon 1 is unlikely to affect the in situ patterns because there is no evidence in either the ENCODE (Additional file 5) or Chromatin State Segmentation data (Figure 6) of a functional promoter between exons 1 and 2.