Skip to main content
Figure 10 | Mobile DNA

Figure 10

From: PGBD5: a neural-specific intron-containing piggyBac transposase domesticated over 500 million years ago and conserved from cephalochordates to humans

Figure 10

Salt extraction of sonicated crude nuclei from mouse brain. (A) Crude nuclei from mouse whole brain were lysed and chromatin sheared by sonication. Nuclear lysis was assayed by DAPI staining before and after sonication (top left panel). Sonication was monitored by agarose gel electrophoresis with and without DNase I digestion (top right panel). (B) Sonicated nuclei were extracted with NaCl at the indicated concentrations (mM) with or without prior DNase I digestion. Supernatant and pellet fractions were separated by centrifugation, resolved by SDS-PAGE, and assayed by western blotting for PGBD5 (top panels) or histone H3 (bottom panels).

Back to article page

Mobile DNA

ISSN: 1759-8753