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Table 1 Plasmids used in the present study

From: The site-specific integration reaction of Listeria phage A118 integrase, a serine recombinase

Plasmid

Descriptiona

Source

pRJ2184

pET15b, A118 integrase between Nde I-Bam H1

This work

pRJ2186

pET11a, A118 integrase between Nde I-Bam H1

This work

pRJ2823

pET15b, A118 integrase158-452 between Nde I-Bam H1

This work

pRJ2214

pBR322, attP (400 bp) into Eco R1

This work

pRJ2215

pBR322, attB (200 bp) into Sal I

This work

pRJ2289

pBR322, attPU153 (200 bp) into Eco R1

This work

pBCPB+

pBCSK+, cam (lacZ)

Groth and colleagues [50]

pIntA1

pACYC177, kan lacP-A118 integrase

Keravala and colleagues [31]

pBCPB-A1+

pBCSK+, cam DR(attB-lacZ-attP)

Keravala and colleagues [31]

pRJ2799

pBCPB+, cam IR(attB-lacZ-attP)

This work

pRJ2825

pBCPB+, cam DR(attB(G1C)-lacZ-attP)

This work

pRJ2826

pRJ2825, attP (G1C)

This work

pRJ2827

pBCPB+, cam DR(attB(G2C)-lacZ-attP)

This work

pRJ2828

pRJ2827, attP (G2C)

This work

pRJ2829

pBCPB+, cam DR(attB(A3T)-lacZ-attP)

This work

pRJ2830

pRJ2829, attP (A3T)

This work

pRJ2831

pBCPB+, cam DR(attB44-lacZ-attP)

This work

pRJ2927

pBCPB+, cam DR(attB41-lacZ-attP)

This work

pRJ2832

pBCPB+, cam DR(attB39-lacZ-attP)

This work

pRJ2833

pBCPB+, cam DR(attB37-lacZ-attP)

This work

pRJ2834

pBCPB+, cam DR(attB35-lacZ-attP)

This work

pRJ2835

pBCPB+, cam DR(attB-lacZ-attP50)

This work

pRJ2836

pBCPB+, cam DR(attB-lacZ-attP48)

This work

pRJ2848

pBCPB+, cam DR(attB-lacZ-attP46)

This work

pRJ2837

pBCPB+, cam DR(attB-lacZ-attP44)

This work

pRJ2928

pBCPB+, cam DR(attB-lacZ-attP42)

This work

pRJ2913

pBCPB+, cam DR(attL-lacZ-attR)

This work

Strain

DP-L 3689 (10403::A118)

R Calendar

DP-L 3670 (10403S::U153)

R Calendar

DP-L 4056 (10403S phage cured)

R Calendar

  1. aatt sites are from A118 unless specified. DR, att sites in direct orientation; IR, att sites in inverted orientation.