Figure 6

Integrase binding to half - att sites. (A) Sequences of attP half-sites (Po- and -oP’). Lowercase letters designate non-att sequences on the duplex probes. (B) Gel mobility shift assay with the Po- half-site. Left panel contains increasing concentrations of integrase (0, 10, 30, 90, 270, and 810 nM), and right panel shows binding with 810 nM of the C-terminal integrase domain. (C) Gel mobility shift assay with the -oP’ half-site using 810 nM of integrase or the C-terminal domain. (D) Sequences of attB half-sites (Bo- and -oB’). (E) Gel mobility shift assay with the Bo- half-site. Left panel contains increasing concentrations of integrase (0, 10, 30, 90, 270 and 810 nM), and right panel shows binding with 810 nM of the C-terminal domain. (F) Gel mobility shift assay with the -oB’ half-site using 810 nM of integrase and the C-terminal domain.